Phenolic profile and antioxidant activity of date-pits of seven Algerian date palm fruit varieties

Abstract

The total phenolic content of methanol and ethyl-acetate extracts of pits from seven Algerian palm date (PhOEnix dactylifera L.) fruit varieties was estimated by the Folin-Ciocalteau method. Their respective antioxidant activities were evaluated by the DPPH center dot and the Co(II)-EDTA luminol chemiluminescence tests and their phenolic profile was established by LC-DAD-MS (ESI and ESI+). The total phenolic content of the methanolic extracts ranged from 27.2 to 38.5 mg of caffeic acid equivalents CAE/100 g fresh weight, while the ethyl acetate extracts gave contents ranging from 22.8 to 42.6 mg CAE/100 g fresh weight. The antiradical and the hydroxyl scavenging activities of the methanolic extracts were much higher than those of the ethyl acetate extracts. The different varieties that were studied did not show any major difference in their phenolic profile. Indeed, all the varieties contained mainly catechin or epi catechin derivatives, sinapic, cinnamic, and coumaric acid derivatives. However, it should be noted that, unexpectedly, a glycosylated aurone was tentatively identified in the Tazizaout variety. Further investigations are required to characterise the non-identified flavonoids.