Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well characterized human genital organ culture
Authors & affiliation
Lennert Steukers, Steven Weyers, Xiaoyun Yang, Annelies Vandekerckhove, Sarah Glorieux, Maria Cornelissen, Wim Van Den Broeck, Marleen Temmerman, Hans Nauwynck
Abstract
We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were found to be expressed in all tissues during the entire menstrual cycle. HVEM expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaquewise mucosal spread across the basement membrane and induced prominent epithelial syncytia.
Publication date:
2014
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Attachments
J_Infect_Dis.-2014-Steukers-infdis_jiu036.pdf (restricted)Related publications
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